WebEverquest Quest Information for ToL Rank II Level 120 Spells: Glowing Etched Bloodstone WebNov 1, 1999 · Abstract. TrfA is the only plasmid-encoded protein required for RK2 replication. We report here the construction and characterization of an RK2-based vector in which trfA is expressed from the inducible promoter Pm.The resulting construct, pJBSD1, was found to replicate in Escherichia coli DH5a (recA −) only in the presence of a Pm …
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